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Accession Number 5004877

Title Sediment Quality in Canals that Discharge into Biscayne Bay, Dade County, Florida

Project Description Contaminants discharged into Biscayne Bay, FL from freshwater canals in Dade County have

degraded habitat quality and may be adversely impacting fish and wildlife resources. Sources of

contamination to the Bay will be identified through chemical analyses and toxicity testing of

solid-phase sediments and sediment pore waters. Approximately 100 sediment samples from the

canals will be shipped to Patuxent Wildlife Research Center Athens for toxicity testing and

analysis. Sediments will be analyzed for metals, PAHs, PCBs and organochlorine pesticides.

Sediment particle size, percent organic matter, acid volatile sulfides (AVS) and simultaneously

extracted metals (SEM) will be measured from each sediment. Toxicity will be determined by

exposing Hyalella azteca (freshwater amphipod) to solid-phase sediments for 28 d under

static-renewal conditions and to sediment pore water for 96 h under static conditions. Survival and

growth will be test endpoints for solid-phase exposures and survival for porewater exposures.

Toxicity of pore water with differing levels of oxidation and methanol extractions from sediments

and will also be determined using the Microtox bioassay with the photo-luminescent bacterium,

Photobacterium phosphoreum. Identification of areas contributing contaminants to Biscayne Bay

will form the basis of management strategies developed to enhance and protect habitat quality in

the Bay. Data will also provide insight into the factors influencing concordance/discordance of

toxicity between solid-phase sediment toxicity and porewater toxicity. Information gained from

comparisons between tests of sediments and pore waters and the significance of porewater

oxidation on toxicity will contribute directly to the process of methods standardization for

porewater testing.

Keywords contaminants, metals, organic pollutants, pollution, pore water, sediment, sediment quality,

toxicity,

Principal Parley V Winger, USGS Patuxent Wildlife Research Center: parley_winger@usgs.gov; Peter J

Investigators Lasier, USGS Patuxent Wildlife Research Center: pete_lasier@usgs.gov;

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